The three strains (XM2, 265, and 842) and one commercial additive (L) were used as inoculants and singularly added to the native grass. plantarum, Pediococcus acidilactici, and Latilactobacillus graminis, by sequencing 16S rRNA, respectively. Strains XM2, 265, and 842 could grow normally at 15☌–30☌, pH 4.0–8.0, and NaCl 3 and 6.5% they were identified as Lactiplantibacillus plantarum subsp. The effect of selected strains on bacterial community and quality of native grass silage was also studied. This study aimed to isolate and identify lactic acid bacteria (LAB) from the native grass and naturally fermented silage from the Mongolian Plateau. 2National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Molecular Nutrition, Ministry of Education, Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Nutrition and Feed Science of Zhejiang Province, Institute of Feed Science, Zhejiang University, Hangzhou, China.1Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.Sihan You 1†, Shuai Du 2†, Gentu Ge 1*, Tao Wan 1* and Yushan Jia 1*